The EPS yield (240 mg L−1) was 45% higher than previously reported. In this study, we evaluated: (i) the increasing production of EPS by Bacillus licheniformis B3-15 in the novel SG17 medium (ii) the arsenic absorption capacity of the EPS by mass spectroscopy (iii) the functional groups of EPS interacting with As by ATR-FTIR spectroscopy and (iv) the ability of EPS to prevent arsenic toxicity by the bioluminescent assay. The EPS-producing Bacillus licheniformis B3-15, of shallow marine vent origin (Vulcano Island, Italy), was previously reported as tolerant to arsenate (AsV). Exopolysaccharide (EPS) production represents an adaptive strategy developed by extremophiles to cope with environmental stresses.
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